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rabbit anti-human/mouse phospho-creb (s133)  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti-human/mouse phospho-creb (s133)
    Rabbit Anti Human/Mouse Phospho Creb (S133), supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti-human/mouse phospho-creb (s133)/product/Cell Signaling Technology Inc
    Average 90 stars, based on 1 article reviews
    rabbit anti-human/mouse phospho-creb (s133) - by Bioz Stars, 2026-03
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    Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated <t>CREB</t> in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.
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    Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated <t>CREB</t> in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.
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    Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated <t>CREB</t> in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.
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    Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated <t>CREB</t> in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.
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    Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated CREB in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.

    Journal: Journal of advanced research

    Article Title: A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization.

    doi: 10.1016/j.jare.2025.04.034

    Figure Lengend Snippet: Fig. 1. Sauchinone possesses TGR5 agonizing activity. (A) The structure of Sauchinone. (B) Mouse or human TGR5/CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or INT-777. The TGR5 agonist activity was calculated by determining the luciferase activity in cell lysate. (C) CRE/HEK293 stable cell lines were incubated with different concentrations of Sauchinone or Forskolin. The CRE agonist activity was calculated by determining the luciferase activity in cell lysate. (D) Human FXR/FXRE/Huh7 cells were incubated with different concentrations of Sauchinone or OCA. The FXR agonist activity was calculated by determining the luciferase activity in cell lysate. (E) The thermal stability of TGR5 protein in BMDM and THP-1 cells treated with or without Sauchinone (30 lM) was measured by CETSA. (F) BMDM or THP-1 cells were incubated with different concentrations of Sauchinone for 1 h. The protein level of phosphorylated CREB in cell lysates was detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05 compared as indicated.

    Article Snippet: Anti-mouse STAT-1 (9712L), anti-mouse p-STAT-1 (7649L), antimouse Inducible nitric oxide synthase (INOS) (13120S), Anti- mouse STAT-6 (9362S), anti-mouse p-STAT-6 (56554S), antimouse PKA (5842S), anti-mouse p-CREB (9197S), anti-mouse CREB (9198L),anti-mouse p-IjB (9246L), anti-mouse IjB (4812S), antimouse p-p65 (3033L), anti-mouse p65 (4764S), anti-mouse ASC (67824S) and anti-mouse NLRP3 (15101S) were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Activity Assay, Stable Transfection, Incubation, Luciferase, Western Blot

    Fig. 6. Sauchinone normalizes TGR5/PKA/CREB pathway and regulates M1/M2 macrophages markers in skin of IMQ-induced psoriasis mice. (A) The protein levels of TGR5, PKA. p-CREB, CREB in skin tissues were detected by western blot. (B) Cytokines including IL-6, IL-12 TNF-a, IFN-c, IL-17 and IL-10 levels in serum were measured by ELISA (n = 6). (C) Cytokines including IL-6, IL-12, TNF-a, IFN-c, IL-1b and IL-10 levels in skin homogenates were measured by ELISA (n = 6). (D) The mRNA levels of M2 markers including Ym-1, Cd-301, Dectin-1, Arg-1, Irf-4 and Ppar-c in skin tissues were measured by qPCR (n = 6). (E) The protein levels of M1 markers including p-STAT-1, STAT1, p-p65, p65, NLRP3, p-IjB, IjB, Pro Caspase-1 and ASC in skin tissues were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

    Journal: Journal of advanced research

    Article Title: A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization.

    doi: 10.1016/j.jare.2025.04.034

    Figure Lengend Snippet: Fig. 6. Sauchinone normalizes TGR5/PKA/CREB pathway and regulates M1/M2 macrophages markers in skin of IMQ-induced psoriasis mice. (A) The protein levels of TGR5, PKA. p-CREB, CREB in skin tissues were detected by western blot. (B) Cytokines including IL-6, IL-12 TNF-a, IFN-c, IL-17 and IL-10 levels in serum were measured by ELISA (n = 6). (C) Cytokines including IL-6, IL-12, TNF-a, IFN-c, IL-1b and IL-10 levels in skin homogenates were measured by ELISA (n = 6). (D) The mRNA levels of M2 markers including Ym-1, Cd-301, Dectin-1, Arg-1, Irf-4 and Ppar-c in skin tissues were measured by qPCR (n = 6). (E) The protein levels of M1 markers including p-STAT-1, STAT1, p-p65, p65, NLRP3, p-IjB, IjB, Pro Caspase-1 and ASC in skin tissues were detected by western blot. Data were shown as Mean ± SEM from triplicate measurements. *P < 0.05, **P < 0.01 compared as indicated.

    Article Snippet: Anti-mouse STAT-1 (9712L), anti-mouse p-STAT-1 (7649L), antimouse Inducible nitric oxide synthase (INOS) (13120S), Anti- mouse STAT-6 (9362S), anti-mouse p-STAT-6 (56554S), antimouse PKA (5842S), anti-mouse p-CREB (9197S), anti-mouse CREB (9198L),anti-mouse p-IjB (9246L), anti-mouse IjB (4812S), antimouse p-p65 (3033L), anti-mouse p65 (4764S), anti-mouse ASC (67824S) and anti-mouse NLRP3 (15101S) were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

    Fig. 9. Schematic diagram of how Sauchinone regulates macrophage polarization through targeting TGR5 and ameliorates IMQ induced psoriasis. Sauchinone inhibits TNF- a + IFN-c induced macrophage M1 polarization in TGR5 dependent way and promotes IL-4 + IL-13 induced M2 polarization in TGR5 independent way. Mechanistically, Sauchinone agonizes TGR5 on macrophage and induces CREB phosphorylation to inhibit NF-jB activation induced M1 polarization. TGR5 antagonist SBI-115 or PKA inhibitor H89 abolishes the influence of Sauchinone on M1 polarization. Moreover, Sauchinone shows potential pharmacological activity on IMQ induced psoriasis by regulating macrophage polarization and inhibiting keratinocyte activation.

    Journal: Journal of advanced research

    Article Title: A novel TGR5 agonist Sauchinone ameliorates IMQ induced murine psoriasis by regulating macrophage polarization.

    doi: 10.1016/j.jare.2025.04.034

    Figure Lengend Snippet: Fig. 9. Schematic diagram of how Sauchinone regulates macrophage polarization through targeting TGR5 and ameliorates IMQ induced psoriasis. Sauchinone inhibits TNF- a + IFN-c induced macrophage M1 polarization in TGR5 dependent way and promotes IL-4 + IL-13 induced M2 polarization in TGR5 independent way. Mechanistically, Sauchinone agonizes TGR5 on macrophage and induces CREB phosphorylation to inhibit NF-jB activation induced M1 polarization. TGR5 antagonist SBI-115 or PKA inhibitor H89 abolishes the influence of Sauchinone on M1 polarization. Moreover, Sauchinone shows potential pharmacological activity on IMQ induced psoriasis by regulating macrophage polarization and inhibiting keratinocyte activation.

    Article Snippet: Anti-mouse STAT-1 (9712L), anti-mouse p-STAT-1 (7649L), antimouse Inducible nitric oxide synthase (INOS) (13120S), Anti- mouse STAT-6 (9362S), anti-mouse p-STAT-6 (56554S), antimouse PKA (5842S), anti-mouse p-CREB (9197S), anti-mouse CREB (9198L),anti-mouse p-IjB (9246L), anti-mouse IjB (4812S), antimouse p-p65 (3033L), anti-mouse p65 (4764S), anti-mouse ASC (67824S) and anti-mouse NLRP3 (15101S) were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Phospho-proteomics, Activation Assay, Activity Assay